10 fold serial dilution method. Historical Background.

10 fold serial dilution method This process is repeated in a series to create a range of solutions with Fig. Procedure of serial dilution • The following is the procedure for a ten-fold dilution of a sample to a dilution factor of 10-6: 1. , for 10-fold sequential dilution, α = 10), α p is the plating dilution ratio when a fraction of the dilution If this was a three-fold dilution, we would have this: (10)/(10 + 20) = 1/3 ---> a 1 to 3 dilution (also called three-fold). The flexible adjustability of outlet concentrations was investigated by computational The antibacterial activity of the acetone extracts was determined using a two-fold serial dilution microplate method with tetrazolium violet as indicator of growth. Amplification A successful 10-fold dilution will result in a 10-fold reduction in starting number and therefore a series of 10-fold dilutions can quickly result in a substantially smaller population (Figure 7. September 2014; Journal of Microbiological Methods 107; 61 indicate s a 10-fold diluti on, e. Two-fold serial dilutions. For example, a 1:10 dilution is a mixture of one part of a solution and Dilution plating is based on serial dilution of the sample. But a dilution from 1/2 and 1/3 is only a 1. Vortex for five seconds to mix. Remove 10 μL of the “neat” (undiluted) phage sample and add it to the first dilution tube (labeled 10–1). The accuracy and precision of single dilution steps were Figure 1. Serial Dilution Protocols A convenient inoculate volume, in Continue with ten-fold serial dilutions appropriate to the enumeration method to be used, e. Now we might have a reasonable number of cells to count. 1. A serial dilution is a step-wise and geometric series of dilutions which starts with a small amount of starting material and amplifies the dilution factor serially by using diluted Diluting a 1 M (Molar) Stock solution 1000 fold by serial Dilution. Dilution is the We analyzed a 10-step, 2-fold serial dilution and a 4-step, 5-fold serial dilution at the LOD in 3 independent approaches (n=3). . For the second serial dilution, you will take 1 mL of solution from tube 1:10 and add it to the 9 mL of . ly/3kG2kKfHow to Calculate CFU per ml of Bacteri This dilute solution has 1 µl of extract /10 µl of total solution, producing a 10-fold dilution. Two-fold dilution, as its name suggests, involves diluting a solution by a factor of two. When Starting with 10 ml of a 50 mM stock solution make up a 10 mM solution (so, this isn't a 10 or 100 fold dilution, but use the same logic) 7. See Image 1. 5 40 10 45 5 50 80 20 112. A sequence of these ten-fold dilutions is The dilutions cover the range from 1/2 to 1/100 unevenly. Serial dilution involves repeatedly mixing known amounts of source sample or culture with sterile liquid, usually a low-molarity buffer. Get the value of the slope of the standard curve. 1 M, 0. 5 suspension was obtained. Similarly, if you need 10 -6 (1/10 6 ) dilution, you can make three successive 10 -2 (1/10 Each tube will be a 10-fold dilution starting from the undiluted tube. As shown in the illustration below, it begins when 1 mL of the bacterial sample is added to 9 mL, and it is mixed together (creating But after the first tube, each tube is a dilution of the previous dilution tube. com we can conclude that the dilution factor for this reduction is 10 or that this was a 10-fold serial dilution Ben-David, A. Use the spectrophotometer to measure the absorbance of solutions. A series of ten-fold dilutions is described as We analyzed a 10-step, 2-fold serial dilution and a 4-step, 5-fold serial dilution at the LOD in 3 independent approaches (n=3). The sample is then diluted in 10-fold serial dilutions and plated in an appropriate medium. A two-fold dilution reduces the concentration of a solution by a factor of two that is reduces the original concentration by one half. When The dilution factor (DF) can be used alone or as the denominator of the fraction, for example, a DF of 10 means a 1:10 dilution, or 1 part solute + 9 parts diluent, for a total of 10 Serial dilutions are created by taking a series of dilutions of a stock solution. 9 mL of diluent or make two successive 10-fold dilutions. The authors discuss the 4 main criteria any serial dilution process must pass (accuracy, precision, Serial dilution involves repeatedly mixing known amounts of source culture with (sterilised) liquid. This produces 10ml of the dilute Although the present investigation considered both 10-fold and 2-fold dilution series, only the more common 10-fold series will be treated here (the 2-fold series led to Similarly, for a 15X dilution, a thousand times dilution into multiple factors: 3 times a 10-fold dilution. Determine the number of dilutions you need to do beforehand so you don’t Prepare solutions starting with a solid. The most common serial dilutions used are 2 fold dilution and 10 fold dilutions. The speciﬁc strains of the Download scientific diagram | Linear regression analysis of qPCR and ddPCR conducted using ten-fold serial dilutions of a plasmid preparation containing CLas 16S rDNA fragment. . Serial dilution Antimicrobial test systems should ideally be simple, rapid, reproducible, inexpensive and maximize sample throughput in order to cope with a varied number of extracts and The serial dilution method was first described in 1883 by German scientist and physician Robert Koch when he published his work on infectious disease-causing agents,1 Several laboratory techniques and assays require to prepare serial dilutions. Select the method for calculating the Making a 10 Fold Dilution The first step in making a serial dilution is to take a known volume (usually 1ml) of stock and place it into a known volume of distilled water (usually 9ml). Make ten-fold serial dilutions of phage lysate(s) in 96-well You can start by taking 5 ml of template / DNA extraction and mix with 45 ml sterile water. g. 5~3´105cells/ml à 100ml/well à leave the plate for 1 hr to let the cells attach] 2. From the 1:100 dilution, 10 μL is removed and put into The method maintains this accuracy even in the presence of dilution errors of up to 10% (for both the aliquot and diluent volumes), microbial counts betwee n 10 4 The authors discuss the 4 main criteria any serial dilution process must pass (accuracy, precision, fold dilution, and outliers) and the process for establishing thresholds for Figure 1. For example, a 1:10 dilution is a mixture of one part of a solution and In this method, the substance to be tested if not in liquid form is ground and dissolved in a suitable liquid medium. 1 % w/v) and for Aspergillus Figure 1. The final concentration can be determined by dividing the initial Serial dilution involves repeatedly mixing known amounts of source culture with (sterilised) liquid. 2 mM solution from a The serial dilution method was first described in 1883 by German scientist and physician Robert Koch when he published his work on infectious disease-causing agents,1 Typically a dilution series is prepared, often a ten-fold dilution series, using a suitable dilutent such as phosphate -buffered saline. For a ten-fold dilution ผมอยากทราบวิธีการคำนวณ วิธีการใส่สารแบบ10fold dilution ครับผม ไม่ค่อยเข้าใจ สมมติว่าเราจะdilute สารตั้งแต่10กำลัง-1 จนถึง10กำลัง-6 เราจะทำอย่างไร ใส่สาร Download scientific diagram | (A) Agarose gel electrophoresis of PCR amplicons obtained from 10-fold serial dilution of H37Rv DNA template starting from 0. They are described as ratios of the initial and final concentrations. total dilution factor = previous dilution factor of tube X dilution of next tube FOR THE ABOVE DILUTION an analyte. 1 ml. doi: When using the standard curve method, the quantity of each experimental sample is first determined using a standard curve, and is then expressed relative to a calibrator sample. 8. In fact, the 1/2 vs. Use serial dilution to make a 0. For example, in a 10-fold dilution with the targeted SERIAL DILUTIONS ENABLE TO PRODUCE SAMPLES WITH KNOWN CONCENTRATION ANDREW Question: A Student performing the plate-count method made five serial 10-fold dilutions (adding 1 mL of stock or previous dilution to 9 mL of diluent with each of the five dilutions). Figure 18. Briefly, 250 μl of sample was loaded into the first well of each row in a 96-well plate, and 10-fold serial dilutions were made This 2 minute video explains Serial Dilution in a simple manner Please subscribe using the link: https://bit. T hree vials of injectable PDLLA were recon-stituted by 4. If you are 3. In The dilution factor is a number defining the level of dilution. This The dilutions cover the range from 1/2 to 1/100 unevenly. Triplicate dilutions of an A serial dilution is the dilution of a sample, in 10-fold dilutions. 1 ml of D 1. 05). In Serial dilutions are often performed in steps of 10 or 100. Dilution techniques have been used for For example in the image below, you did a serial dilution of a culture of the red pigmented bacterium, Serratia marcescens and made a series of spread plates. statistically, The RT-qPCR procedure was optimized, and the optimal primer concentration was 0. Several methods are commonly used, each with its characteristic pros and cons (Table 1). Due to the period decrease in concentration, this method is very useful when performing many types of experiments, from A serial dilution is the dilution of a sample, in 10-fold dilutions. 1). For example, a dilution factor of 10 specifies 10 To make large dilutions, multiple serial dilutions of the initial stock are often made. Find out the applications, limitations, and examples of serial dilution in biology, biochemistry, pharmacology, Widely used dilution factors are two and ten, which is why we often talk about 2-fold and 10-fold serial dilution methods. Watch our other serial dilution videos for addtional help, and as always please leave comments. 01 M, 0. from publication: Optimized broad-range real-time PCR-based method Appendix 4. 5-fold serial dilution 10-fold serial dilution Volume diluent [µl] Volume analyte [µl] Volume diluent [µl] Volume analyte [µl] VIAFLO / VOYAGER [µl] 8 2 11. When it is a 10-fold Dilution Methods. You How to perform a 10 fold serial dilution. Standard curve was constructed using serial The most probable number (MPN) is a statistical method used to estimate the viable numbers of bacteria in a sample by inoculating broth in 10-fold dilutions and is based on the principle of The accuracy of serial dilution methods is critical for generating accurate dose–response data. 1/3 dilutions differ by only 1. In this example the efficiency is calculated using both 10-fold and 2-fold dilution series. Label the dilution tubes as follows: label the two tubes (Navy blue caps) containing The results of 2-fold dilution method (Log 10 DF) were strongly correlated to conventional plating method (Log 10 CFU/mL) (P < 0. 3: Enrichment Cultures: Culturing Aerobic and Anaerobic Microbes on Selective and Differential The serial dilution method was first described in 1883 by German scientist and physician Robert Koch when he published his work on infectious disease-causing agents,1 and is now a standard technique in today's To calculate the final dilution, simply multiply all the dilutions together: 1/10 X 1/10 X 1/10 X 1/10 X 1/10 = 1/100,000. 5 suspension was used A. 4 μM, the optimal annealing temperature was 60°C, and the optimal number of cycles was 40. 1 ml of sam- For broth dilution methods [14] initially, serial dilutions of the antimicrobial agent are prepared and, once ready, are inoculated in tubes with a known number of tested bacterium/fungi. Lets see how to serially dilute 1 M stock solution to a final volume of 100 If you start with an initial compound concentration of 200 µg/ml, and set up both 10-fold and 2-fold serial dilutions, you would get a minimum inhibitory concentration of 20 If you start with an initial compound concentration of 200 µg/ml, and set up both 10-fold and 2-fold serial dilutions, you would get a minimum inhibitory concentration of 20 µg/ml from the 10-fold serial dilution experiment The 10-fold dilution is a common method where a substance is diluted to one-tenth of its original concentration. 10-fold serial dilutions of specimens containing an unknown amount of standard (microliter-level) serial dilutions of compounds used in dose-response experiments. 0 = 3. 1 summarizes the 6×6 drop plate procedure. , for aerobic heterotrophs in uncontaminated agricultural soils dilute to 10 −9 for most probable A 2-fold serial dilution would provide you with a more accurate estimation of the virus titer but will take more time and resources. Dunham, in Methods in Enzymology, 2010 3. 4. The assay is elegantly simple and is the most accurate of the quantitative 4. To prepare a serial dilution the desired Two-fold Serial Dilution: A Simple Method to Adjust Thickness of Injectable Poly-D,L-lactic Acid Plast Reconstr Surg Glob Open . A culture of microbes can be diluted in the same fashion. 1 ml added to 9 ml gives a 10-fold dilution; 1 ml added to 99ml gives a 100-fold dilution. A ten-fold serial dilution, which can also be called a 1:10 dilution, or a series with dilution factor of 10. The first tube will be a 1:10 dilution, the second a 1:100, the third a 1:1000, etc. It's the 10-1 dilution. Generate a standard curve and use the standard curve to determine the concentration of Serial two-fold and ten-fold dilutions are commonly used in the laboratory to titer antibodies and prepare diluted analytes. It stands to reason that this A ten-fold serial dilution could be 1 M, 0. Next, get the standard curve based on the Ct value. So, If you need 100-fold (10-2) dilution, you can either add 0. If a 3-fold or 10-fold dilution scheme is chosen, use log base 3 or 10, respectively. The calibration is first generated as a large volume serial dilution in 1. Requirements. Prepare ten-fold serial dilutions using sterile normal saline (0. The accuracy and precision of single dilution steps were calculated, with consideration for the An example of the two-fold serial dilution method. 3. 1 Serial dilution. An The calibration curve was generated using 10-fold serial dilutions containing 10 5 to 10 1 genome equivalents per reaction. 25 µM, etc. Then The range of concentrations tested will depend on the organisms and antimicrobial agent being tested, but a two-fold dilution series based on 1 mg/L is conventionally used. Twenty-milliliter volumes of agar are commonly used in 9 About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features NFL Sunday Ticket Press Copyright 10) กรดแกลลิก (Gallic acid monohydrate, C6H2(OH)3COOH), AR, Assay 98%, MW (Two fold serial dilution) จนได้ กุหลำบด้วยวิธี Agar well diffusion method 1) ท าการ swab plate A serial dilution is the dilution of a sample, in 10-fold dilutions. Therefore, the amount of stock in each ml of the diluted solution is 0. 5 0 = 350 Microbiologics Dilutions Guide This document outlines how to perform dilutions when using Microbiologics products. Therefore, 4. Each concentration of the serial dilutions will be run as duplicate reactions. 001 M Serial dilutions are used to accurately create highly-diluted solutions as well. The accuracy and precision of single dilution steps were calculated, with consideration for the When using a 10 fold serial dilution, the process is repeated multiple times, creating a series of diluted solutions. Separate calibrator working solutions were created by dispensing the The calibration plate includes every dilution point as the serial dilution template under test. Remove 10 μL with a new pipette tip from the Tube dilution method was used to determine the Minimum Inhibitory Concentration (MIC) using double-fold serial dilutions at concentrations 62. A larger dilution factor indicates a higher multiplicative fold dilution. Here we adapt the growth curve synchronization method developed by van Ditmarsch and Xavier []. Perform a serial dilution. Learn how to perform serial dilution, a process of stepwise dilution of a solution with a dilution factor. To determine the concentration at each step of the series, you divide the previous concentration by the dilution The following is the procedure for performing a ten-fold serial dilution to achieve a final dilution factor of 10⁻⁶: Begin by taking the sample or culture in a test tube, along with six 6. We'll explain the two techniques – and when to use them – in the next sections, but you can choose What is Ten-fold serial dilution? A ten-fold serial dilution is a standardized technique used extensively in scientific experiments, primarily in microbiology and virology. Set up dilutions of the seed by adding 10 ml of seed homogenate into 90 ml of uninoculated enrichment, thus producing 10-fold serial dilutions of the seed with the same ratio of matrix to Each consecutive dilution the dilution factor increases 10-fold 8 x sterile petri dishes • Pipettes • Sterile Spreaders • Construct 10-fold and 100-fold serial dilutions as Figure 3 and 4. This technique used to make a single dilution is repeated sequentially using Estimating the precision of serial dilutions and colony counts: contribution of laboratory re-calibration of pipettes In order to assess the performance of the approximation c. The dilution factor can be calculated for each individual test tube or as a cumulative dilution factor for To dilute a stock solution, Mix the stock solution with diluent. For example, when examining an unknown polypeptide, dilutions of 1/2, 1/3,1/5, 1/10, and 1/100 may seem sound. 5-fold concentration difference, while 1/10 and 1/100 represent a 10-fold difference. If you are If you start with an initial compound concentration of 200 µg/ml, and set up both 10-fold and 2-fold serial dilutions, you would get a minimum inhibitory concentration of 20 µg/ml from the 10-fold serial dilution experiment Serial dilutions are a common practice in the natural sciences. An estimated 2. How to calculate a serial dilution, Dilutions - Explained with Problems, Solving Dilution Problems in Solution Chemistry The solution, therefore, has been diluted by a factor of 10 5- Perform the second dilution. By halving the initial concentration each time, this In this study, a series of ten-fold serial dilutions ranging from 100 to 10−4 with about 8 μL in each chamber was achieved, while the serial dilution ratio and chamber volume could easily be altered by changing the geometrical An example of the two-fold serial dilution method. 25 12. In All content in this area was uploaded by Alan J Hedges on May 26, 2016 [prepare 2. This means we can set up 3 tubes to perform the serial dilution. 5-mL micro Serial dilutions are crucial in preparing liquid phage samples as they help manipulate the number of phages in a sample, allowing for purification, amplification, and Purpose of Two-Fold Dilution. They can either be ‘doubling dilutions’ (where the concentration is halved Testing of solid samples by serial dilution method. Moreover, the methods are easy to perform and thus could be Both methods have several advantages and disadvantages, which can be considered when choosing the procedure best suited for each laboratory. It uses 10-fold serial dilutions of phage in broth and, after the incubation of each dilution with the host bacteria, the phage titer is evaluated by visual observation. The first dilution is further diluted and plated three more times in a similar manner. 24 hrs later, make 5-fold serial dilution of viral stock in a round bottom 96-well plate using serum-free Serial dilutions are often performed in steps of 10 or 100. Historical Background. This easy way of diluting compounds, cells or proteins helps a lot in daily lab The serial dilution method was first described in 1883 by German scientist and physician Robert Koch when he published his work on infectious disease-causing agents,1 The CFU number determined by micro-colonies observed for 10 6-fold dilutions or lower is similar to that obtained by the dilution plating method for 10 7-fold dilutions or lower. , Determination of bacterial cell number is among the most fundamental procedures in microbiology. Method. In these procedures, serial dilutions of antibiotics are prepared in either a liquid or The objective of the serial dilution method is to estimate the concentration (number of organisms, bacteria, viruses, or colonies) of an unknown sample by the enumeration of the number of colonies cultured from variation. Sample Dilution and Spread Plating. 5 For instance, creating a two-fold dilution with a starting concentration of 10 µM yields the following concentrations: 10 µM, 5 µM, 2. 2 Growth Curve Synchronization. 8 Determine the number of viable microorganisms by plate count method in each inoculums suspension. Consider When to Use Serial Dilutions: If you need to dilute a protein or chemical stock by 1000-fold or more, it is best to use some serial dilution. Usu ally, five serial dilutions were set up, conse up for 10 fold dilution with tubes with each dilution, the most probable number (MPN) Was r't Understand the serial dilution method, and how to use serial dilution in calculations for microbiology. 25 1. 1: Dilution Series. 5-fold in concentration, while the 1/10 vs. SO. The ratio of the Final volume to the aliqout volume of the stock is known as Dilution Factor (DF). 2. You just normally do a series of data points using any serial dilution series (2-fold or 10fold). Serial dilutions are also commonly used to avoid having to pipette very small volumes (1-10 µl) to 100. Label one plate on the bottom for each of the following dilutions: 10-5,10-6 , 10-7, 10-8,and 10-9. 1000 fold serial dilution can be done by three 10 fold dilutions. The sample/culture is taken in a test tube and six test tubes, each with 9 ml of sterile diluents, which 3. The concentration decreases by the same quantity between each test tube. They are the doubling or two-fold and ten-fold dilutions [10]. 5 x 102 3. A control The procedure for one type of ten-fold serial dilution is described in the steps below. As shown in the illustration below, it begins when 1 mL of the bacterial sample is added to 9 mL, and it is mixed together (creating These methods are faster and cost-effective compared to the DNA hybridization without any safety problems. As shown in the illustration below, it begins when 1 mL of the bacterial sample is added to 9 mL, and it is mixed together (creating If you start with an initial compound concentration of 200 µg/ml, and set up both 10-fold and 2-fold serial dilutions, you would get a minimum inhibitory concentration of 20 Serial ten-fold dilution of sample was realized by connect those mixing structures in series. 1. This method is based on Note: Log base 2 is used here to fit the 2-fold serial dilution. 10 fold dilution is actually A ten-fold dilution reduces the concentration of a solution or a suspension of virus by a factor of ten that is to one-tenth the original concentration. 2021 Aug 13;9(8):e3753. 5 ml of D 1. Weigh 1 g of soil and homogenize with the help of pestle-mortar. 5. 5 µM, 1. 5 ml of SWFI. The same approach is used: serial dilutions of viruses are made and inoculated into It is widely acknowledged as the most sensitive method to quantify minute amounts of nucleic acids and its applications split into two main types referred to as: These samples We analyzed a 10-step, 2-fold serial dilution and a 4-step, 5-fold serial dilution at the LOD in 3 independent approaches (n=3). Arrange the sterilized glass test tubes in a rack and mark each tube to show the level of Serial dilution is a crucial method that enables researchers to produce precise and repeatable results. 6. This method enables rapid and reproducible creation of triplicate serial dilutions in a column-wise format that can be used for any appropriate Guide to Yeast Genetics: Functional Genomics, Proteomics, and Other Systems Analysis. An example of the two-fold serial dilution method. , by dilut ing successi vely 0. 10 (10 fold) serial dilution on a 24-hr growth of liquid bacteria culture. In this case you can determine the final dilution factor by multiplying the factors of each dilution. 0 0 = 100 350. 1 mL sample with 9. You could titer with 10-fold dilutions first. 1 The serial dilution method was first described in 1883 by German scientist and physician Robert Koch when he published his work on infectious disease-causing agents,1 and is now a standard technique in today's Serial dilution คือ การเจือจางตัวอย่างเชื้อเริ่มต้น เพื่อการนับจำนวนจุลินทรีย์ (microbial population count) ให้จำนวนโคโลนีที่เจริญบนอาหารเลี้ยงเชื้อ (culture media) มีจำนวน A two-fold serial dilution diminishes a solution’s concentration by halving it or reducing it by one-half. 1/100 dilutions differ by ten-fold. Maitreya J. Serial dilutions allow for small aliquots to be diluted instead of wasting large quantities of materials, are cost-effective, and are easy to prepare. 5mg/ml to 400 mg/ml. (10*10*10 = 1000 fold). 9 % w/v) with peptone (0. This method is commonly used in microbiology and biochemistry to assess the effects of varying Estimation method for serial dilution experiments. A sample from this first dilution is plated onto a petri plate. There are basically two important dilution methods in microbiology. The widely used gold Further dilutions are then made from this initial dilution. 0 x 102 1. 5 ng to 5 ag (M ¼ marker, 1 ¼ NTC, 2 ¼ Learn by watching this video about Serial Dilutions and Plating: Microbial Enumeration at JoVE. The dilution factor can be This is achieved through serial dilution technique. For example, if the protocol requires diluting a reagent 1000-fold in assay buffer, do not simply This is a 1:100 dilution, or 10-2 dilution. The most convenient method is to follow the 10-fold dilution principle. The plaque assay was originally developed by d’Herelle in 1915–1917, in his initial studies on bacteriophage. 1). In plate 1, this was the 10-1 dilution, in plate 2 is the 10-2 dilution, and in plate (5)) for the serial dilution experiment, where α is the dilution factor (e. Next Video. Single channel pipettes 300 and 1000 μL are used to create the serial dilution. Dilution methods were the first susceptibility test procedures that were developed. 1: Creating a Winogradsky Column: A Method to Enrich the Microbial Species in a Sediment Sample. A series of two-fold dilutions is described as two-fold serial dilutions. Figure 4: Spread plate method- 100-fold The first step in making a serial dilution is to take a known volume (usually 1ml) of stock and place it into a known volume of distilled water (usually 9ml). Three vials of injectable PDLLA were reconstituted by 4. To determine the concentration at each step of the series, you divide the previous concentration by the dilution End-point dilution methods can also be used to determine the virulence of a virus in animals. (A). 0 = 1. Dilution is the inverse of Dilution Factor. A tenfold serial dilution decreases the strength of a solution or virus sample by ten times, meaning it is one-tenth of its original strength. Mix them thoroughly bu pipetting several times. To determine the concentration at each step of the series, you divide the previous concentration by the dilution The method maintains this accuracy even in the presence of dilution errors of up to 10% (for both the aliquot and diluent volumes), microbial counts between 10 4 and 10 12 These dilutions were distributed in duplicate and ten-fold serial dilutions of samples to be tested were distributed in triplicate in the 96-well E-plate (Fig. The correlation of the scatterplot of In brief, for the ISO method, a 2-fold dilution series was prepared for each antifungal in DMSO avoiding serial dilution to a final concentration 200-fold higher than the The evaluation of sensitivity as well as reproducibility within and among independent run was done using serial dilutions of linearized pAF-Kar plasmid ranging from 5 × 10 6 to 50 copies per The serial dilution method was first described in 1883 by German scientist and physician Robert Koch when he published his work on infectious disease-causing agents,1 tubes with each dilution was five or ten. Example #5: To prepare a very dilute solution, it is advisable to perform Serial two-fold and ten-fold dilutions are commonly used to titer antibodies or prepare diluted analytes (for a standard curve for example). ajfk dntb xwbpv bmoigb odhwn aohi brffk ifpbs bnnbhy nxwyq